{"id":1564,"date":"2024-09-17T15:04:00","date_gmt":"2024-09-17T12:04:00","guid":{"rendered":"https:\/\/pediatria-spisanie.eu\/2024\/09\/17\/kletuchna-diagnostika-na-maligneni-eksudati-pri-deca\/"},"modified":"2025-05-18T22:03:12","modified_gmt":"2025-05-18T19:03:12","slug":"kletuchna-diagnostika-na-maligneni-eksudati-pri-deca","status":"publish","type":"post","link":"https:\/\/pediatria-spisanie.eu\/en\/2024\/09\/17\/kletuchna-diagnostika-na-maligneni-eksudati-pri-deca\/","title":{"rendered":"Cellular Diagnosis of Malignant Exudates in Children"},"content":{"rendered":"\n\n\t\t
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M. Yordanova, B. Avramova, T. Kamenska, N. Yurukova, K. Bachvarov, I. Vlahova, T. Hristova, I. Boronsuzov, Y. Doncheva, N. Kartulev, Z. Antonova, H. Shivachev <\/h3>\n

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Abstract<\/h2>\n\n\n\n

The urgent intervention for drainage of massive effusions of unknown origin in children and adolescents
has a therapeutic decompressive effect and concurrently provides the potential for rapid clinical orientation and diagnosis. Routine immunological cell typing using flow cytometry is not a standardly accepted investigation but is a fast and reliable method for identifying effusions associated with underlying malignant diseases. The results of 16 immunophenotypic investigations for primary diagnosis of pleural and peritoneal exudates in 9 pediatric patients with malignant diseases – 8 with hematologic neoplasms and one with a solid tumor – were retrospectively reviewed. The sensitivity, characteristics, and limitations of the method, as well as its correlation with the final diagnosis, are discussed.
Key words:<\/strong> malignant effusions; pediatric age; cytology; flow cytometry<\/p>\n\n\n

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Bibliography<\/strong><\/h2>\n\n\n\n
    \n
  1. DeCamp MM, Jr., Metzer SJ, Swanson SJ, Sugarbaker DJ. Malignant effusive disease of the pleura and pericardium. Chest 1997; 112:291S\u2013295S.<\/li>\n\n\n\n
  2. \u041a\u043e\u0441\u0442\u043e\u0432 \u041a. \u0414. \u041a\u0430\u043b\u0435\u0432. \u041f\u043b\u0435\u0432\u0440\u0430\u0442\u0430. \u0422\u0435\u043a\u0441\u0442\u043e\u0432\u0435 \u0437\u0430 \u043a\u043b\u0438\u043d\u0438\u0447\u043d\u0430 \u0438 \u043d\u0430\u0443\u0447\u043d\u0430 \u043f\u0440\u0430\u043a\u0442\u0438\u043a\u0430. \u0418\u0437\u0434. \u201d\u0410\u041d\u041a\u041e\u0421\u201d, \u0421\u043e\u0444\u0438\u044f, 2005<\/li>\n\n\n\n
  3. Helson L, Krochmal P, Hajdu SI. Diagnostic value of cytologic specimens obtained from children with cancer. Ann Clin Lab Sci 1975;5:294\u2013297, Wong JW, Pitlik D, Abdul-Karim FW. Cytology of pleural, peritoneal and pericardial fluids in children. A 40-year summary. Acta Cytol 1997; 47:467\u2013473).<\/li>\n\n\n\n
  4. Motherby H. et al.  Diagnostic accuracy of effusion cytology. Diagn Cytopathol 1999; 20: 350\u2013357.<\/li>\n\n\n\n
  5. Yam LT, et al. Immunocytochemical diagnosis of lymphoma in serous effusions. Acta Cytol 1985; 29:833\u2013841)<\/li>\n\n\n\n
  6. Katz RL, et al. A morphologic, immunologic, and cytometric approach to the classification of non-Hodgkin\u2019s lymphoma in effusions. Diagn Cytopathol 1987;3:91\u2013101)<\/li>\n\n\n\n
  7. Yu GH. et al. Use of Flow Cytometry in the Diagnosis of Lymphoproliferative Disorders in Fluid Specimens. Diagnostic Cytopathology 2014, Vol. 42, No 8.<\/li>\n\n\n\n
  8. Das DK. Serous Effusions in Malignant Lymphomas: A Review. Diagnostic Cytopathology, 2006; Vol 34, No 5.  <\/li>\n\n\n\n
  9. 9. Chang A. et al. Lineage-Specific Identification of Nonhematopoietic Neoplasms by Flow Cytometry. Am J Clin Pathol 2003; 119: 643-655; Pillai V. et al. A Simplified Flow Cytometric Immunophenotyping Procedure for the Diagnosis of Effusions Caused by Epithelial Malignancies. Am J Clin Pathol 2013; 139:672-681.<\/li>\n\n\n\n
  10. Davidson B. Molecular testing on serous effusions. Diagn Cytopathol.2021; 49(5):640-646).<\/li>\n\n\n\n
  11. Pinto D. et al. The international system for reporting serous fluid cytopathology\u2014diagnostic categories and clinical management, Journal of the American Society of Cytopathology, Volume 9, Issue 6, 2020, 469-477.<\/li>\n\n\n\n
  12. Fuller MY, Thrall MJ. Utility of flow cytometry analysis for pleural and peritoneal fluids, Journal of the American Society of Cytopathology 2016,<\/li>\n\n\n\n
  13. Wong JW, Pitlik D, Abdul-Karim FW. Cytology of pleural, peritoneal and pericardial fluids in children. A 40-year summary. Acta Cytol 1997;47:467\u2013473.<\/li>\n\n\n\n
  14. van der Velden et al. Sample preparation and staining in VHJ EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012 Sep;26(9):1986-2010.<\/li>\n\n\n\n
  15. Stein H, et al. CD30+ anaplastic large cell lymphoma: a review of its histopathologic, genetic, and clinical features. Blood (2000) 96 (12): 3681\u20133695.<\/li>\n\n\n\n
  16. Kao EY, et al. ALK Negative Anaplastic Large Cell Lymphoma. In: StatPearls. Treasure Island(FL): StatPearls Publishing; 2024 Jan. https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK519019<\/a>.<\/li>\n\n\n\n
  17. Craig<\/a> FE., Foon KA. <\/a>Flow cytometric immunophenotyping for hematologic neoplasms. Blood (2008) 111 (8): 3941\u20133967.<\/li>\n\n\n\n
  18. Flow Cytometry and Effusions in Lymphoproliferative Processes and Other Hematologic Neoplasias Beata Bode-Lesniewska Acta Cytologica 2016; 60:354\u2013364.<\/li>\n\n\n\n
  19. Bhaker P, et al. Precursor T-lymphoblastic lymphoma: speedy diagnosis in FNA and effusion cytology by morphology, immunochemistry, and flow cytometry. Cancer Cytopathol 2015; 123: 557\u2013 565.<\/li>\n\n\n\n
  20. McGowan P, et al.  Differentiating between Burkitt lymphoma and CD10+ diffuse large B-cell lymphoma: the role of commonly used flow cytometry cell markers and the application of a multiparameter scoring system. Am J Clin Pathol. 2012 Apr;137(4):665-70. <\/li>\n\n\n\n
  21. Gabali A. Serous fluids and hematolymphoid disorders. Cytojournal. 2022 Mar 19;19:17.<\/li>\n\n\n\n
  22. 22. \u041f\u0435\u043d\u0435\u0432\u0430 \u041c. \u041f\u043b\u0435\u0432\u0440\u0430\u043b\u043d\u0438 \u0438\u0437\u043b\u0438\u0432\u0438 – \u0434\u0438\u0444\u0435\u0440\u0435\u043d\u0446\u0438\u0430\u043b\u043d\u043e \u0434\u0438\u0430\u0433\u043d\u043e\u0441\u0442\u0438\u0447\u0435\u043d \u043f\u043e\u0434\u0445\u043e\u0434. \u0421\u044a\u0440\u0446\u0435 – \u0431\u044f\u043b \u0434\u0440\u043e\u0431, \u044f\u043d. 2003, \u0431\u0440\u043e\u0439 1, \u0441\u0442\u0440. 16-28.<\/li>\n<\/ol>\n\n\n
    <\/div>\n\n\n\n

    Address for correspondence:<\/strong><\/p>\n\n\n\n

    <\/p>\n\n\n\n

    KDKHO, UMBAL \u201cTsaritsa Yoanna-ISUL\u201d,
    8, \u201cBjalo More\u201d, Str.
    1527, Sofia, Bulgaria
    m.jordanova@sbaldohz.com<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    M. Yordanova, B. Avramova, T. Kamenska, N. Yurukova, K. Bachvarov, I. Vlahova, T. Hristova, I. Boronsuzov, Y. Doncheva, N. Kartulev, Z. Antonova, H. Shivachev<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_pediatria_doi":"","footnotes":""},"categories":[1301,1339],"tags":[1680,1681,1682,1683,1347,1684,1685,1686,1687,1688,1689,1690,1691,1692,1693,1694,1695,1696,1697],"class_list":["post-1564","post","type-post","status-publish","format-standard","hentry","category-articles","category-issue-3-2024","tag-flow-cytometry-en","tag-malignant-effusions-en","tag-pediatric-age-cytology-en","tag-b-avramova-en","tag-detska-vazrast-en","tag-z-antonova-en","tag-i-boronsuzov-en","tag-i-vlahova-en","tag-j-doncheva-en","tag-k-bachvarov-en","tag-kletachna-diagnostika-na-maligneni-eksudati-pri-decza-en","tag-m-jordanova-en","tag-n-kartulev-en","tag-n-yurukova-en","tag-potochna-czitometriya-en","tag-t-kamenska-en","tag-t-hristova-en","tag-h-shivachev-en","tag-czitologiya-en"],"_links":{"self":[{"href":"https:\/\/pediatria-spisanie.eu\/en\/wp-json\/wp\/v2\/posts\/1564","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pediatria-spisanie.eu\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pediatria-spisanie.eu\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pediatria-spisanie.eu\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pediatria-spisanie.eu\/en\/wp-json\/wp\/v2\/comments?post=1564"}],"version-history":[{"count":1,"href":"https:\/\/pediatria-spisanie.eu\/en\/wp-json\/wp\/v2\/posts\/1564\/revisions"}],"predecessor-version":[{"id":1566,"href":"https:\/\/pediatria-spisanie.eu\/en\/wp-json\/wp\/v2\/posts\/1564\/revisions\/1566"}],"wp:attachment":[{"href":"https:\/\/pediatria-spisanie.eu\/en\/wp-json\/wp\/v2\/media?parent=1564"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pediatria-spisanie.eu\/en\/wp-json\/wp\/v2\/categories?post=1564"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pediatria-spisanie.eu\/en\/wp-json\/wp\/v2\/tags?post=1564"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}